Pressure-Driven Sample Flow through an Electrospun Membrane Increases the Analyte Adsorption

Electrospun polymer membranes are regarded as prospective biosensor components due to their large specific surface area and diverse opportunities for chemical modifications. However, intricate porous structure can impede diffusion render some analyte-binding sites inaccessible. To overcome these limitations improve analyte adsorption onto the polymer, a pressure-driven sample flow through membrane be employed. date, efficiency of delivery into has not been quantified. Here, we compare forced passive poly(dioxanone) electrospun membranes. We examine two model analytes, BSA interleukin-1 beta (IL1b), address both non-specific binding. Following exposure test solutions, measured residual concentrations analytes using fluorometry enzyme-linked immunosorbent assay (ELISA) techniques. The loading was superior diffusion, with 2.8–11.5-fold change physical 2.4–3.4-fold difference Our data useful development immunoassays microfluidic devices.